From Donor to Patient: Collection, Preparation and Cryopreservation of Fecal Samples for Fecal Microbiota Transplantation

Fecal Microbiota Transplantation (FMT) is suggested as an efficacious therapeutic strategy for restoring intestinal microbial balance, and thus for treating disease associated with alteration of gut microbiota.FMT consists of the administration of fresh or frozen fecal microorganisms from a healthy donor into the intestinal tract of diseased patien

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[Accepted, unedited version] Network analysis for identifying potential anti-virulence targets from whole transcriptome of Pseudomonas aeruginosa and Staphylococcus aureus exposed to certain anti-pathogenic polyherbal formulations

Introduction: Antimicrobial Resistance (AMR) is a serious global threat.Identification of novel antibacterial targets is urgently warranted to help the antimicrobial drug Wide Brim Sun Hat discovery programmes.This study attempted identification of potential targets in two important pathogens Pseudomonas aeruginosa and Staphylococcus aureus.Methods

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The length of vesicular stomatitis virus particles dictates a need for actin assembly during clathrin-dependent endocytosis.

Microbial pathogens exploit the clathrin endocytic machinery to enter host cells.Vesicular stomatitis virus (VSV), an enveloped virus with bullet-shaped virions that measure 70 x 200 nm, enters cells by clathrin-dependent endocytosis.We showed previously Gas Lens Collet Body that VSV particles exceed the capacity of typical clathrin-coated vesicles

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Mechanisms contributing to inhibition of retinal ganglion cell death by cell permeable peptain-1 under glaucomatous stress

Abstract This study assesses the neuroprotective potential of CPP-P1, a conjugate of an anti-apoptotic peptain-1 (P1) and a cell-penetrating peptide (CPP) in in vitro, in vivo, and ex vivo glaucoma models.Primary retinal ganglion cells (RGCs) were subjected to either neurotrophic factor (NF) deprivation for 48 h or endothelin-3 (ET-3) treatment for

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